6,883 research outputs found

    Membrane receptors of mouse leukocytes. II. Sequential expression of membrane receptors and phagocytic capacity during leukocyte differentiation

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    Analysis of four mature cell markers on mouse bone marrow leukocytes grown in vitro, demonstrated a distinct sequence of marker appearance during the terminal phases of granulocytic cell differentiation. A similar pattern of marker expression was also suggested by analysis of mature neutrophils and macrophages isolated from normal tissues. Among cultured neutrophils, receptors for the Fc portion of IgG (FcR) were first expressed on myelocytes and metamyelocytes, and then subsequently on more mature cells. Morphologically mature colony neutrophils (polymorphs) from agar cultures contained only FcR and complement receptor type two (CR(2)) (C3d receptor), and lacked both complement receptor type one (CR(1)) (C3b receptor) and the capacity to ingest latex, bacteria, or iron particles. Neutrophils from 2 and 3 wk liquid media cultures of marrow cells differed from agar grown neutrophils in that they had phagocytic capacity (particle ingestion) [Pi] in addition to FcR and CR(2). Furthermore, in the 4th and 5th wk of these continuous liquid cultures, CR(1) was also expressed, completing the surface marker profile of normal blood neutrophils. Based on these studies, the following order of appearance of these four markers on cells from the myelocytic series was proposed: FcR {arrow} FcR CR(2) {arrow} FcR CR(2) Pi {arrow} FcR CR(2) Pi CR(1). Differential studies of tissue leukocytes containing these same markers revealed that a heterogeneity existed among morphologically mature neutrophils. Even though 95 percent of blood polymorphs contained all four markers, the same was true of only half of spleen polymorphs and only 20 percent of bone marrow polymorphs. Cells of the monocyte-macrophage series were studies in parallel with neutrophils. Cultured marrow monocytes acquired the four mature cell markers so rapidly that the order of receptor appearance could not be determined. However, it was found that CR2 was lost during the terminal phase of monocyte maturation into activated macrophages

    Demographic characteristics of exploited tropical lutjanids: a comparative analysis

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    Demographic parameters from seven exploited coral reef lutjanid species were compared as a case study of the implications of intrafamily variation in life histories for multispecies harvest management. Modal lengths varied by 4 cm among four species (Lutjanus fulviflamma, L. vitta, L. carponotatus, L. adetii), which were at least 6 cm smaller than the modal lengths of the largest species (L. gibbus, Symphorus nematophorus, Aprion virescens). Modal ages, indicating ages of full selection to fishing gear, were 10 years or less for all species, but maximum ages ranged from 12 (L. gibbus) to 36 years (S. nematophorus). Each species had a unique growth pattern, with differences in length-at-age and mean asymptotic fork length (Lāˆž), but smaller species generally grew fast during the first 1ā€“2 years of life and larger species grew more slowly over a longer period. Total mortality rates varied among species; L. gibbus had the highest mortality and L. fulviflamma, the lowest mortality. The variability in life history strategies of these tropical lutjanids makes generalizations about lutjanid life histories difficult, but the fact that all seven had characteristics that would make them particularly vulnerable to fishing indicates that harvest of tropical lutjanids should be managed with caution

    Population genetic data for 17 Y STR markers from Benghazi (East Libya)

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    The seventeen Y-STR loci included in the AmpFā€˜STR1 YfilerTM PCR Amplification kit (DYS19, DYS389I,DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438, DYS439, DYS437, DYS448, DYS458,DYS456, DYS635, and Y-GATA-H4) were used to type a sample population of 238 males from eastern Libya (Benghazi region). Of 238 observed haplotypes, 214 were unique (90%) and 24 (10%) were found more than once. The 17 loci gave a discriminating power of 0.999. DYS458 showed the highest diversity as a single-locus marker (0.73). Allelic frequencies and gene diversities for each Y-STR locus were determined. The high haplotype diversity and discrimination capacity (0.996) demonstrate the utility of these loci for human identification in forensic applications. Comparative analysis with Y-STR datasets of relevant populations and submission of the haplotypes to the Y-STR Haplotype Reference Database (YHRD) was undertaken

    Implementing Pharmacy Informatics in College Curricula: The AACP Technology in Pharmacy Education and Learning Special Interest Group

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    Many professional organizations have initiatives to increase the awareness and use of informatics in the practice of pharmacy. Within education we must respond to these initiatives and make technology integral to all aspects of the curriculum, inculcating in students the importance of technology in practice. This document proposes 5 central domains for organizing planning related to informatics and technology within pharmacy education. The document is intended to encourage discussion of informatics within pharmacy education and the implications of informatics in future pharmacy practice, and to guide colleges of pharmacy in identifying and analyzing informatics topics to be taught and methods of instruction to be used within the doctor of pharmacy curriculum

    Modelling the response of surface fuel to climate change across south-eastern Australia: consequences for future fire regimes

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    Geophysical Research Abstracts of EGU General Assembly 2014, held 27 April - 2 May, 2014 in Vienna, Austria

    A Gravitational Redshift Determination of the Mean Mass of White Dwarfs. DBA and DB Stars

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    We measure apparent velocities (v_app) of absorption lines for 36 white dwarfs (WDs) with helium-dominated atmospheres -- 16 DBAs and 20 DBs -- using optical spectra taken for the European Southern Observatory SN Ia progenitor survey (SPY). We find a difference of 6.9+/-6.9 km/s in the average apparent velocity of the H-alpha lines versus that of the HeI 5876AA for our DBAs. This is a measure of the blueshift of this He line due to pressure effects. By using this as a correction, we extend the gravitational redshift method employed by Falcon et al. (2010) to use the apparent velocity of the HeI 5876AA line and conduct the first gravitational redshift investigation of a group of WDs without visible hydrogen lines. We use biweight estimators to find an average apparent velocity, _BI, (and hence average gravitational redshift, _BI) for our WDs; from that we derive an average mass, _BI. For the DBAs, we find _BI = 40.8+/-4.7 km/s and derive _BI = 0.71 +0.04 -0.05 Msun. Though different from of DAs (32.57 km/s) at the 91% confidence level and suggestive of a larger DBA mean mass than that for normal DAs derived using the same method (0.647 +0.013 -0.014 Msun; Falcon et al. 2010), we do not claim this as a stringent detection. Rather, we emphasize that the difference between _BI of the DBAs and of normal DAs is no larger than 9.2 km/s, at the 95% confidence level; this corresponds to roughly 0.10 Msun. For the DBs, we find ^He_BI = 42.9+/-8.49 km/s after applying the blueshift correction and determine _BI = 0.74 +0.08 -0.09 Msun. The difference between ^He_BI of the DBs and of DAs is less than or equal to 11.5 km/s (~0.12 Msun), at the 95% confidence level. The gravitational redshift method indicates much larger mean masses than the spectroscopic determinations of the same sample by Voss et al. (2007)...Comment: Accepted to the Astrophysical Journal, 10 pages double-column, 3 figures, 5 table
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